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Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

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HANGZHOU EASTBIOPHARM CO.,LTD.
HANGZHOU EASTBIOPHARM CO.,LTD.
City: Hangzhou
Province/State: Zhejiang
Tel: 86-571-86483186
Contact Person:
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Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

Place of Origin : china
Brand Name : eastbiopharm
Model Number : CK-E30082
Certification : COA,ISO9000
Price : 300USD/KIT
Packaging Details : BOXING
Delivery Time : 3DAYS
Payment Terms : T/T Western Union MoneyGram
Supply Ability : 500KITS/WEEK
MOQ : 1KIT
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Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

Please carefully read this instruction before using. This ELISA kit is based on the principle of double-antibody sandwich technique to detectRat soluble cluster of differentiation 14,sCD14 ELISA KitBe used only for research purposes, not be used for medical diagnosis.


Intended Use:
This kit is used to assay the sample ofSerum, blood plasma,Saliva, Urine, andother related tissue Liquid.


Test principle

The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level ofsCD14samples. AddsCD14to monoclonal antibody Enzyme well which is pre-coated withsCD14monoclonal antibody, incubation; then, addsCD14antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finallybecomes yellow. The chroma of color and the concentration of the SubstancesCD14elisa kit of sample were positively correlated.


Materials supplied in the Test Kit


1

Standard

0.5ml

7

Chromogen Solution A

6ml

2

Standard diluent

3ml

8

Chromogen Solution B

6ml

3

Microelisa Stripplate

12w×8s

9

Stop Solution

6ml

4

Str- HRP-Conjugate Reagent

6ml

10

Instruction

1

5

30×wash solution

20ml

11

Closure plate membrane

2

6

Biotin-Ab

1ml

12

Sealed bags

1


Materials required but not supplied

1. 37 °C incubator

2. Standard Enzyme reader

3. Precision pipettes and Disposable pipette tips

4. Distilled water

5. Disposable tubes for sample dilution

6. Absorbent paper


Summary Procedure

Preparing reagents, samples and standards

Add prepared samplesand standards,antibodies labeled with enzyme, reacting60 minutes at 37°C

Plate washedfive times, adding Chromogensolution A, B, reacting10 minutes at 37°C

Add stop solution

Measurethe OD valuewithin 10min


Package size:96T per box.

Validity&Storage:Six months(2-8°C)orTwelvemonths(-20°C)

[see label on the outer box for the specific date].

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